Do we need to use laboratory analysis to confirm the result?
Rapid Cassette Test kits do not need any confirmation from laboratory analysis. They provide an immediate qualitative response.
The COVID-19 IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma) is a qualitative membrane-based immunoassay for the detection of IgG and IgM antibodies to COVID-19 in whole blood, serum or plasma specimen.
This test consists of two components, an IgG component and an IgM component. In the IgG component, anti-human IgG is coated in IgG test line region. During testing, the specimen reacts with COVID-19 antigen-coated particles in the test cassette. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with the anti-human IgG in IgG test line region, if the specimen contains IgG antibodies to COVID-19. A colored line will appear in IgG test line region as a result of this.
Similarly, anti-human IgM is coated in IgM test line region and if specimen contains IgM antibodies to COVID-19, the conjugate-specimen complex reacts with anti-human IgM. A colored line appears in IgM test line region as a result. Therefore, if the specimen contains COVID-19 IgG antibodies, a colored line will appear in IgG test line region. If the specimen contains COVID-19 IgM antibodies, a colored line will appear in IgM test line region. If the specimen does not contain COVID-19 antibodies, no colored line will appear in either of the test line regions, indicating a negative result. To serve as a procedural control, a colored line will always appear in the control line region, indicating that the proper volume of specimen has been added and membrane wicking has occurred